Manipur University

LEIMAPOKPAM TIKENDRA SINGH

Designation: Guest Faculty

Specialization: Plant Biotechnology

Contact: +91 9862767469

Email: tikendra [AT] manipuruniv.ac.in

PhD

LEIMAPOKPAM TIKENDRA SINGH, M.Sc., Ph.D

Guest Faculty

Department of Biotechnology

Manipur University, Imphal-795003, India

tikendra [AT] manipuruniv.ac.in; Contact: +91 9862767469

07/2019 – Present

EXPERIENCE ACADEMIC Guest Faculty) Manipur University, India

Guest Faculty at Department of Biotechnology

2015-2020 RESEARCH Manipur University, India

Plant tissue culture

Developing in vitro propagation protocols for conservation of orchids and other medicinal plants.
Studying various effect of phytohormone on the growth and development of plants.
Genomic DNA extraction of the micropropagated plants and assessing the genetic stability using PCR based molecular markers.

Population genetic study

Developing conservation strategies by studying the genetic variation within and among the population, genetic differentiation, gene flow and population genetic structure from different population of the orchids.
Assessing genetic parameters and genetic pattern distribution.

Synthetic seeds preparation

Protocol standardization for encapsulation of in vitro raised shoot propagules with

protective nutrient gel which provides another promising option for mass propagation.

2012-2015 PROJECT FELLOW Manipur University, India

Establishment of plant tissue culture laboratory
Developing in vitro propagation protocol for the bamboo, Dendrocalamus latiflorus.

EDUCATION

PhD in Plant Biotechnology MANIPUR UNIVERSITY

Master’s Degree in Biotechnology HNB GARHWAL UNIVERSITY

Bachelor’s Degree in Biology UNIVERSITY of DELHI

ADDITIONAL DEGREES

AWARD

Succeeded Graduate Aptitude Test in Engineering (GATE), 2013, organized Indian Institute of Technology Bombay on behalf of the National Co-ordination Board (NCB)-GATE for the Department of Higher Education, Ministry of Human Resource Development (MHRD), Government of India.
Succeeded State Level Eligibility Test for Lectureship, North-East Region, 2013, accredited by University Grants Commission, New Delhi, India.
Completed “Post Graduate Diploma in Sustainability Science”, School of Agriculture, Indira Gandhi National Open University (IGNOU), New Delhi, India.

SKILLS

Experience in handling plant tissue culture.
Experience in genomic DNA extraction and downstream experiments.
Experience in protein extraction from plants and isoenzyme characterization.
Electrophoresis (Agarose gel and PAGE), PCR.
Chromosomal staining for ploidy analysis.

RESEARCH

PUBLICATIONS
Statistical software such as- POPGENE, MEGA, GenALEx, Excel, STRUCTURE, GENELAND

First author publications

Cytokinin influence on in vitro shoot induction and genetic stability assessment of Dendrocalamus latiflorus Munro: a commercially important bamboo in Manipur, North-East India. Vegetos, 2022. https://doi.org/10.1007/s42535-022-00392-5

Tikendra L, Dey A, Imlitoshi Jamir, Manas Ranjan Sahoo, Nongdam P.

Micropropagation of Bamboos and Clonal Fidelity Assessment Using Molecular Markers. Biotechnological Advances in Bamboo. Springer, Singapore, 2021. https://doi.org/10.1007/978-981-16-1310-4_7.

Tikendra L, Choudhary R, Sanayaima Devi R, Dey A, Potshangbam AM., Nongdam P.

RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.- an important endangered orchid. Physiology and Molecular Biology of Plants, 2021. https://doi.org/10.1007/s12298-021-00939-x

Tikendra L, Potshangbam AM, Dey A, Devi TR, Sahoo MR, Nongdam P.

Understanding the genetic diversity and population structure of Dendrobium chrysotoxum Lindl.-an endangered medicinal orchid and implication for its conservation. South African Journal of Botany, 2021, 138:364-376

Tikendra L, Potshangbam AM, Amom T, Dey A, Nongdam P.

Dendrobium sp.: In vitro propagation of genetically stable plants and ethnomedicinal uses. In: Merillon JM, Kodja H (eds) Orchids Phytochemistry, Biology and Horticulture. Reference Series in Phytochemistry. Springer, Cham. 2021. https://doi.org/10.1007/978-3-030-11257-8_30-1

Tikendra L, Apana N, Potshangbam AM, Amom T, Choudhary R, Sanayaima R, Dey A, and Nongdam P.

Molecular genetic homogeneity assessment of micropropagated Dendrobium moschatum Sw.- A rare medicinal orchid, using RAPD and ISSR markers. Plant Gene, 2019, 19. doi.org/10.1016/j.plgene.2019.100196

Tikendra L, Amom T, Nongdam P.

Molecular markers based genetic fidelity assessment of micropropagated Dendrobium chrysotoxum Lindl. Meta Gene, 2019, 20. doi.org/10.1016/j.mgene.2019.100562 Tikendra L, Koijam AS, Nongdam P.

Effect of phytohormones on rapid in vitro propagation of Dendrobium thyrsiflorum Rchb. f.: An endangered medicinal orchid. Pharmacognosy Magazine, 2018, 14:495-500.

Tikendra L, Amom T, Nongdam P.

Genetic clonal fidelity assessment of rhizome derived micropropagated Acorus calamus linn. – a medicinally important plant, by RAPD and ISSR markers. Pharmacognosy Magazine (Accepted for publication in 2022).

Tikendra L, Sushma O, Amom T, Apana N, Sonia P, Bidyananda N, Potshangbam AM, Devi RS, Nongdam P.

Co-author publications:

Molecular clonal fidelity assessment of micropropagated orchids using DNA markers.
Biotechnology and Crop Improvement. CRC Press, 2022.

Sonia P, Apana N, Tikendra L, Dey A, Jamir I, Nongdam P.

Molecular markers in assessing genetic clonal fidelity for in vitro propagated endangered medicinal plants. Molecular Genetics and Genomics Tools in Biodiversity Conservation. Springer, Singapore. 2022. https://doi.org/10.1007/978-981-16-6005-4_6

Biswas P, Nandy S, Dey A, Tikendra L, Nongdam P.

Genetic diversity and population structure of Clerodendrum serratum (L.) Moon using CBDP, iPBS and SCoT markers. Journal of Applied Research on Medicinal and Aromatic Plants, 2021.

Apana N, Amom T, Tikendra L, Potshangbam AM, Dey A, Nongdam P.

Efficiency of RAPD, ISSR, iPBS, SCoT and phytochemical markers in the genetic relationship study of five native and economical important bamboos of North-East India. Phytochemistry, 2020, 174.

Amom T, Tikendra L, Apana N, Goutam M, Sonia P, Koijam AS, Potshangbam AM, Rahaman H, Nongdam P.

Polyamine elicited aristolochic acid production in in vitro clonally fidel Aristolochia indica L.: An ISSR and RAPD markers and HPTLC based study. South African Journal of Botany, 2020. https://doi.org/10.1016/j.sajb.2020.06.018

Dey A, Nongdam P, Nandy S, Mukherjee S, Tikendra L, Hazra AK, Pandey DK.

Enhanced bacoside content in polyamine treated in vitro raised Bacopa monnieri (L.) Wettst.

South African Journal of Botany, 2019, 123, 259-269.

Dey A, Hazra AK, Nongdam P, Nandy S, Tikendra L, Banerjee S, Pandey DK.

Evaluation of genetic relationship between 15 bamboo species of North East India based on ISSR marker analysis. Molecular Biology Research Communications, 2018, 7:7-15.

Amom T. Tikendra L, Rahaman H, Angamba P, Nongdam P.

ISSR marker based population genetic study of Melocanna baccifera (Roxb.) Kurz: A commercially important bamboo of Manipur, North-East India. Scientifica, 2017, Volume, 2017, https://doi.org/10,1155/2017/3757238

Nilkanta H, Amom T, Tikendra L, Rahaman H, Nongdam P.

Establishment of Efficient in vitro Culture and Plantlet Generation of Tomato (Lycopersicon esculentum Mill.) and Development of Synthetic Seeds. Journal of Plant Sciences, 2015, 10(1), 15-24

Oceania C, Doni T, Tikendra L, Nongdam P.

The nutritional facts of bamboo shoots and their usage as important traditional foods of Northeast India. International Scholarly Research Notices, 2014. Volume 2014, Article ID 679073, 17 pages. hptt:/dx.doi.org10.1155/2014/679073

Nongdam P, Tikendra L.

Establishment of an efficient in vitro regeneration protocol for rapid and mass propagation of Dendrobium chrysotoxum Lindl. using seed culture. The Scientific World Journal, 2014. Volume 2014, Article ID 740150, 8 pages. http://dx.org/10/2014/740150

Nongdam P, Tikendra L.

PRESENTATIONS, TALKS & WORKSHOPS

“National workshop and Hands-On Training programme in molecular techniques in Biotechnology”, 12^th-18^th Oct. 2012, Department of Biotechnology, Manipur University, India.

Micropropagation of Dendrobium thyrsiflorum Rchb.F. through asymbiotic seed germination” presented at International Conference on “Biotechnological Advances in Environmental Health and Biodiversity Conservation (EHBC)”, organized by Department of Biotechnology, Ministry of Science & Technology, Government of India, 21^st-23^rd May, 2015, Manipur University, India.
“Effect of activated charcoal in asymbiotic seed germination and plantlet development of Dendrobium moschatum” presented at the National seminar on “Emerging crops of North-east India suitable for horticulture based integrated farming system”, organized by Central Agriculture University, Iroisemba, 7^th-9^th Feb. 2017, Manipur, India.
“Capacity building in grant writing skills and effective management of Intellectual Property Rights (IPR) in biotechnology by universities and research institutions in the North-east region”, organized by Biotech Consortium India Limited (BCIL), New Delhi, 20^th-22^nd March, 2017, at Department of Biotechnology, Manipur University, India.
“Molecular markers based clonal fidelity assessment of in vitro raised Dendrobium moschatum, a rare medicinal orchid”, a poster presented at the 105^th Indian Science Congress, held at Manipur University, 16^th to 20^th March, 2018, organized by Ministry of Science & Technology, Government of India.
Participated in World Bamboo Workshop, organized by Government of Manipur, World Bamboo Organization and Dicma Trade, 4^th-8^th Feb. 2019, at Manipur, India.
Attended digital module workshop on “Genes to Pathway”, organized by DELIFE, 5^th -16^th Oct. 2020.
Attended digital module workshop on “Multi Omics Box”, organized by DELIFE, 23r Nov. – 6^th Dec. 2020.